Journal: Biomedical Journal

Article Title: Endothelial activation, cell-cell interactions, and inflammatory pathways in postoperative atrial fibrillation following cardiac surgery

doi: 10.1016/j.bj.2024.100821

Figure Lengend Snippet: Connexins expression and apoptosis level in a representative tissue sample from each type of patient: permanent AF, POAF and non-AF. Cx40: connexin 40; Cx43: connexin 43. Connexins can be seen in brown between the junctions of the cells. Apoptosis occurs when the nuclei of cardiomyocytes appear brown.

Article Snippet: After endogenous peroxidase and unspecific background blocking, sections were incubated with the corresponding primary antibody diluted in blocking solution overnight at 4 °C (anti-Cx40 polyclonal Antibody [36–4900, Invitrogen, dilution 1/100] and anti-Cx43 monoclonal Antibody [13–8300, Invitrogen, dilution 1/250]).

Techniques: Expressing

Journal: Biomedical Journal

Article Title: Endothelial activation, cell-cell interactions, and inflammatory pathways in postoperative atrial fibrillation following cardiac surgery

doi: 10.1016/j.bj.2024.100821

Figure Lengend Snippet: Connexin levels. A. Cx40. B. Cx43. ∗ p < 0.05 ∗∗ p < 0.01 ∗∗∗ p < 0.001.

Article Snippet: After endogenous peroxidase and unspecific background blocking, sections were incubated with the corresponding primary antibody diluted in blocking solution overnight at 4 °C (anti-Cx40 polyclonal Antibody [36–4900, Invitrogen, dilution 1/100] and anti-Cx43 monoclonal Antibody [13–8300, Invitrogen, dilution 1/250]).

Techniques:

Journal: Communications biology

Article Title: AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing.

doi: 10.1038/s42003-025-08162-0

Figure Lengend Snippet: Fig. 3 | Acute structural and electrical functional analysis of CM-AI purified hiPSC-CM monolayers. a Western blot analysis of atrial-specific CM proteins in purified versus unpurified monolayers. CM-AI + Laser purified monolayers (n = 6, Lanes 1–6) showed significant enrichment of Cx40, cTnI, and mlc2a protein expression compared to time and batch-matched non-purified monolayers. b Cx40 protein expression was significantly enriched in laser-purified hiPSC-CM mono- layers compared to non-purified monolayers (0.73 ± 0.13au; n = 6 vs. 0.08 ± 0.01au; n = 3; ****P < 0.0001, unpaired t-test). c cTnI protein expression was significantly greater in laser-purified monolayers compared to non-purified hiPSC-CM atrial monolayers (0.31 ± 0.09au; n = 6 vs. 0.02 ± 0.01au; n = 3; ***P = 0.0009, unpaired t- test). d mlc2a myofilament protein expression was significantly enriched in laser- purified hiPSC-CM monolayers compared to non-purified monolayers (0.16 ± 0.08au; n = 6 vs. 0.03 ± 0.01; n = 3; *P = 0.04, unpaired t-test). e Atrial hiPSC-

Article Snippet: Primary antibodies used include: Cx40 (ThermoFisher; Cat #36-4900), cTnI (Abcam; ab47003), and mlc2a (Synaptic Systems; #311011).

Techniques: Functional Assay, Western Blot, Expressing